You probably already know that in terms of available venture capital, one city leads all the rest, and it's not even close.

San Francisco, predictably, has the largest VC ecosystem of any metropolitan area in America, according to a report released this week from VC funding data firm Pitchbook. What perhaps is surprising, given the progress and growth of other startup ecosystems, is the size of the gap: San Francisco startups have raised $117.6 billion since 2006--around three times as much as those in New York ($43.6 billion) and Silicon Valley's own San Jose ($35.5 billion).

That's not to say things are trending in the right direction for the city. San Francisco was home to an increasing number of VC deals each year from 2010 to 2014. That number dropped from around 1,900 deals in 2014 to 1,700 last year--and only 656 in the first six months of 2016.

Also noteworthy is the trend in San Francisco's notoriously high rent rates. From July 2015 through July of this year, the Bay Area's average rent price dropped by 1.5 percent. So while the San Francisco tech bubble is huge, signs point to it perhaps losing a bit of air. Chicago (#7) was the only other city in the 12 biggest ecosystems to experience a rent decrease during that time frame.

Overall the trends show there's less and less funding to be had. With the exception of Philadelphia (#11), every city in the top 12 highest-funded cities is on pace for fewer VC deals in 2016 than 2015.

A few cities should be especially enticing to startups looking for a seed round: Boston (#4 in overall VC ecosystem size), Seattle (#6), San Diego (#9) and Philadelphia all saw more startups secure their first round of financing in 2015 than in the year before.

Los Angeles (#5), Washington, D.C. (#8), San Diego (#9), Austin (#10) and Atlanta (#12) rounded out the top 12.